Entwicklung eines in vivo-Modells in der Nacktmaus und der NOD-SCID Maus zur Untersuchung der humanen Endometriose

Abstract

Human endometrial tissue grown in nude and NOD-SCID mice seems to be a suitable model to test the effect of different (anti-)hormones or (anti-)angiogenetic factors on the development and the growth of ectopic endometrium fragments.
Furthermore this model allows the investigation of the cell biological mechanisms involved in the very early events of implantation and persistance of the endometrial lesions. Attachment of the fragments occurs already 2 days after implantation and neoangiogenesis derived from the host tissue is observed after 5 days.
The endometriotic lesions represent an intact morphological structure with glandular epitheliums surrounded by stroma up to 14 days in nude mice. Later on, the glands reveal a more cystic appearance and the epithelium cells are more flattened.
A very good morphological appearance of the endometrial fragments is observed in NOD-SCID mice even after 4 weeks of culturing. In conclusion, implantation of human endometrial fragments into nude mice seems to be a promising model to develop new therapeutical concepts like the anti-angiogenetic factors, and the NOD-SCID mice model seems to be suitable for long-term experiments.