Stabilität von Kalziumfluorid ähnlichen Deckschichten auf dem Zahnschmelz unter erosiven Bedingungen in vitro und in situ
Abstract
There is some evidence that topical treatment with high concentrations of fluoride may protect against erosive substance loss of teeth. However, the mechanism and the degree of a possible protection is still unclear. Therefore, aim of this study was to evaluate the loss of KOH-soluble fluoride on enamel under mild erosive conditions in vitro and in situ. From 20 (in vitro) and 30 (in situ) human third molars 3 cylindrical enamel samples each were prepared and polished flat. All specimen were treated with an aminefluoride solution (1% F) for 3 minutes. The amount of KOH-soluble fluoride was measured directly after one hour storage in artificial saliva (in situ and in vitro), after two or four days (in vitro) and two or seven days (in situ) both under erosive conditions. Additionally some samples were used for four days (in vitro) and seven days (in situ) without erosive attacks and served as control. Erosive attacks were performed by storage in (in vitro) or drinking (in situ) a soft drink (pH 2,8) for 30 seconds three times daily. In between the specimen were stored in artificial saliva or were worn in the mouth. KOH-soluble fluoride was analysed. In vitro: Directly after fluoridation the amount of KOH-soluble fluoride was 96, after 2 days 10.4 (pŁ0.001) and after 4 days 6.2 µg/cm² (pŁ0.05). Samples stored for four days without erosive attacks exhibited 16.3 µg/cm² (pŁ0.001). In situ: Directly after fluoridation the amount of KOH-soluble fluoride was 77.2, after 2 days 66.9 (pŁ0.06) and after 7 days 54.1 µg/cm² (pŁ0.001). Samples worn for seven days without erosive attacks exhibited 42.3 µg/cm² (pŁ0.003). Even under mild erosive conditions KOH-soluble fluoride on enamel is lost rapidly in vitro but is much more stable in situ.